The properties of these cells expressing the OCSC markers (CD44+/CD133+) (Fig. 2e, f) and the mRNA level of CD44 and CD133 were also significantly increased in the microenvironment of chemotherapy-challenged cells (Fig. 3a, b), which confirmed again that the chemotherapy treatment can significantly increase the CSC properties of ovarian cancer cells. This evidence concerns the gene PROM1 and ovarian carcinoma.