To assess whether PRC2 silencing is a common mechanism for PROM1 regulation in CD133-negative leukemia and not restricted to MLL-AF9 AML, we analyzed RCH-ACV (non-MLLr Pre-B-ALL) and ML-2 (MLL-AF6 AML) cell lines, which do not express PROM1 or present CD133 on the cell surface (Fig. 1a and Supplementary Fig. 1a). This evidence concerns the gene AFDN and acute lymphoblastic leukemia.