The results of qRT-PCR and western blot showed that the mRNA and protein levels of cyclin D1 and Bcl-2 were significantly decreased while that of Bax were increased in sh-UFC1 transfected NSCLC cells (Fig. 2f, g), indicating that UFC1 is involved in the regulation of cell apoptosis and cell cycle progression in NSCLC cells. The gene discussed is CCND1; the disease is non-small cell lung carcinoma.