The results of the western blot analysis indicate that the upregulation of TNF-α, the p-p65/p65 ratio, and IL-1β in the SDH of the cystitis model following L-TAMS treatment was all significantly inhibited on day 20 when compared with the cystitis group without L-TAMS treatment (Fig. 8a–c). This evidence concerns the gene TNF and chronic cystitis.