HLA-C and neoplasm: Our ProGeo-neo presents a bioinformatic pipeline for mining tumor specific antigens from next-generation sequencing including genomic and mRNA expression data, incorporating latest netMHCpan (v.4.0) to predict the binding information of mutant peptides to class I MHC molecules, achieving MHC-peptides validation at the peptide/protein level by MaxQuant, and checking potential of T-cell-recognization by adding sequencing screening methods.