To confirm the contribution of RhoA-ROCK signaling inhibition by SC to its neuroprotective effect on I/R, we established the OGD/R model in PC12 cells, a cell line derives from a pheochromocytoma of the rat adrenal medulla and can acquire neuron-like properties when exposed to nerve growth factor (20), to mimic the MCAO-reperfusion in vitro [18]. Here, RHOA is linked to hereditary pheochromocytoma-paraganglioma.