The results of serum cystatin C in the hierarchical summary receiver operating characteristic model were β = -0.28 (95%CI:-0.88–0.31, Z = -0.93, P = 0.352); and λ = 3.79, which indicated that the diagnostic value of blood NGAL for CIN was moderate. This evidence concerns the gene CST3 and cervical squamous intraepithelial neoplasia.