To test this hypothesis, we infected MEFs/HA‐Atg14 with S. pneumoniae TIGR4 WT or ∆cbpC in presence or absence of cycloheximide (CHX) for 1, 2, or 3 h, and then, we examined whether the amount of HA‐Atg14 decreased during early infection in a CbpC‐dependent manner. This evidence concerns the gene ATG14 and infection.