Based on the MutSigCV algorithm, the waterfall diagram revealed the integration status of somatic mutations in TCGA bladder cancer, and the results showed that the somatic mutations of TP53, TTN, KMT2D, ARID1A, MUC16, PIK3CA, and RB1 (P<0.001) were higher (Figure 1B). The gene discussed is RB1; the disease is urinary bladder cancer.