HUWE1 and posterior cortical atrophy: Coexpression of Flag-JMJD1A with increasing amounts of GFP-HUWE1 had no effect on levels of Flag-JMJD1A protein in 293T cells (Fig. 4c), and knockdown of HUWE1 in PCa cells had no effect on JMJD1A protein levels (Fig. 4d).