TNF and malignant colon neoplasm: In a study that investigated the anticancer effect of bifidobacteria extracts, a butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans exerted an antiproliferative action on colon cancer cells; for this effect, tumor necrosis factor-α (TNF-α) and nitric oxide (NO) were found to be important [28].