Furthermore, we sequenced WT/AD whole-brain input samples (2 mice each) to verify the enrichment of microglia and astrocytes, and we developed a transcriptome list of well-known cell-type-specific genes for microglia (e.g., Cx3cr1, P2ry12, Tmem119, Aif1, Olfml3, Ccl3, Itgam), astrocytes (e.g., Aldh1l1, Atp1b2, Aqp4, Sox9, Slc4a4, Mlc1), neurons (e.g., Stmn2, Rbfox3, Syt1, Syn1), endothelial cells (Endo) (e.g., Pecam1, Tie1), oligodendrocyte precursor cells (OPC) (e.g., Pdgfra, Cspg4), and oligodendrocytes (Oligo) (e.g., Nfasc, Kndc1). Here, NFASC is linked to Alzheimer disease.