To determine the correlation between IMPA2 expression and mTORC1 activity, we next performed Western blot analysis to examine the phosphorylation status of Akt, an upstream regulator of mTORC1, and mTORC1 proteins in ccRCC cell lines with different cellular migration abilities (Figure 2A). This evidence concerns the gene AKT1 and nonpapillary renal cell carcinoma.