In line with our analysis of the endothelial-specific fraction using the TRAP methodology (Figure 3f), IDO1 mRNA expression was significantly elevated on day 16 in tumor endothelial cells from agonistic CD40 mAb treated mice as compared to expression in tumor endothelial cells before treatment (day 12) and tumor endothelial cells isolated from isotype control treated mice (Supplementary Fig S4A, S4B). This evidence concerns the gene CD40 and neoplasm.