To further clarify whether decreasing Wip1 expression by miR-129-2-3p upregulation was responsible for the restriction of ICC cell proliferation and invasion, we restored Wip1 expression in miR-129-2-3p overexpressing-QBC-939 and RBE cells via cotransfection with Wip1 overexpression plasmid pCMV-Wip1 (Figure 5A, P<0.05). The gene discussed is PPM1D; the disease is intrahepatic cholangiocarcinoma.