Mechanism research found that Bufalin inhibited the activation of MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, thus inhibiting proliferation of GBC-SD cells and reducing the self-renewal ability of gallbladder cancer stem cells, which further increased the radiation and chemotherapy sensitivity, and reduced the recurrence and metastasis of gallbladder cancer. The gene discussed is MET; the disease is gallbladder cancer.