Bufalin significantly inhibited the sphere formation capability of gemcitabine-resistant pancreatic cancer cell line, decreased the expression of CD24 and ESA, and reduced the metastatic ability of pancreatic cancer-bearing nude mice by inhibiting the Hedgehog signaling pathway, thereby inhibited the development of pancreatic cancer stem cells 6. The gene discussed is CD24; the disease is familial pancreatic carcinoma.