In the present study, we used human kidney proximal tubular (HK-2) cells to establish an I/R model in vitro (Huang et al., 2018; Tang et al., 2018), and ALR was knocked down in HK-2 cells by infection with short hairpin RNA (shRNA) lentiviral particles to investigate the effects of ALR on cell apoptosis, mitochondrial dysfunction, and PINK1/Parkin-mediated mitophagy during I/R injury. The gene discussed is GFER; the disease is infection.