Top predicted upstream regulators were Hepatocyte Nuclear Factor 4 Alpha (HNF4A), Cystatin D (CST5), Rapamycin-Insensitive Companion of mTOR (RICTOR), MYC Proto-Oncogene, MYCN Proto-Oncogene, microtubule associated protein tau (MAPT) and Tumor Protein P53 (TP53) (Supplementary Table S3), which further underlines the cancer context of CUGBP1 and miR-574-5p. The gene discussed is MYCN; the disease is cancer.