Cells that express intercellular adhesion molecule 1 (ICAM-1+ cells), sorted from HCC cell lines and human primary HCC tissues, had higher tumorigenicity than their ICAM-1− counterparts.26 Conversely, liver-specific knockdown of ICAM-1 in hepatitis B virus (HBV) transgenic mice led to a reduction in the formation of liver tumours, as well as liver and lung metastases.26 Further investigation showed that ICAM-1 was transcriptionally regulated by Nanog.26 This evidence concerns the gene ICAM1 and hepatocellular carcinoma.