Significant upregulation of lysine-specific demethylase-1 (LSD1) was reported in LGR5+ HCC cells.31 LSD1 reverses mono- and di-methylation of histone H3K4 in the promoter regions of negative regulators of Wnt pathway, including Prickle1 and APC, thereby inhibiting their expression, to promote β-catenin activation and support the CSC properties of LGR5+ cells. This evidence concerns the gene LGR5 and hepatocellular carcinoma.