The differentiated rodent hybrid neuroblastoma NG108-15 neuronal cell line transiently expressing exogenous mouse sequences for specific nAChR subunits was employed as the defined in vitro nerve cell model for investigating global differential gene expression via RNA sequencing (RNA-seq) in response to sustained exposure to sensitizing levels (nM) of Aβ for neurotoxicity. The gene discussed is CHRNA4; the disease is neuroblastoma.