Thus, to confirm the function of BMAL1 in glioblastoma with gain of function experiments, we prepared Adenovirus-vector (Ad-Vector) and Adenovirus-BMAL1 (Ad-BMAL1) constructs (Figure 4A) and then produced high-titer adenoviruses (~1 × 109 pfu/mL) in HEK293A cells (see Materials and Methods section). Here, BMAL1 is linked to glioblastoma.