Building on these and other efforts, we investigate here, for the first time, the influence of flow-induced shear stress on carboplatin resistance and the activity of the EGFR, mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK), extracellular signal-regulated kinase (ERK) pathway in a 3D perfusion model for ovarian cancer. Here, EGFR is linked to ovarian carcinoma.