Although the tumor-infiltrating CD4+ T cell population was not different after immunization (Figure 5B), rlipoE7m plus POCpG/DOTAP combined significantly decreased the number of tumor-infiltrating Tregs (day 3: 15.82 ± 6.24%; day 5: 19.42 ± 4.65%) compared with rlipoE7m plus POCpG (day 3: 30.28 ± 4.03%; day 5: 36.33 ± 1.44%) (Figure 5C). Here, CD4 is linked to neoplasm.