At this stage, the mice were euthanized and the prostate tumors were dissected and digested for isolation of primary PCa cells using fluorescence-activated cell sorting (FACS) of GFP+ CD45− cells, or isolation of tumor-infiltrating Gr-MDSCs using magnetic-activated cell sorting (MACS) of CD11b+ Ly6G+ Ly6Clow cells. The gene discussed is ITGAM; the disease is prostate neoplasm.