In order to evaluate the effect of obesity and exercise training on adipocyte differentiation, protein abundance of biomarkers involved in the adipogenesis was measured, including C/EBP-α, C/EBP-β, PPAR-γ, and a master transcription factor regulating brown adipogenesis PRDM-16. This evidence concerns the gene PRDM16 and obesity due to melanocortin 4 receptor deficiency.