Most importantly, subcutaneous injection of hyperinsulinemic Wistar rats with rapamycin (4 mg/kg b.w.)prevented the hyperinsulinemia-induced serine phosphorylation of IRS-1 and the reduction in the IR/IRS-1/PI3K/Akt signaling pathway in muscle and liver tissues [14]. Here, IRS1 is linked to Hyperinsulinemia.