Most importantly, subcutaneous injection of hyperinsulinemic Wistar rats with rapamycin (4 mg/kg b.w.)prevented the hyperinsulinemia-induced serine phosphorylation of IRS-1 and the reduction in the IR/IRS-1/PI3K/Akt signaling pathway in muscle and liver tissues [14]. The gene discussed is IRS1; the disease is hyperinsulinism.