Furthermore, the metabolic profile and overall health was significantly improved by reducing adipose tissue lipolysis, hepatic steatosis, intrahepatic content, triglycerides content, inflammation markers (IL-6 and TNF-α) and, most importantly, increasing mitochondrial efficiency by activation of AMPK, PGC-1α and SIRT1 in muscle, thereby mimicking the effects of calorie restriction [72]. The gene discussed is PPARGC1A; the disease is fatty liver disease.