When GPC1-specific hCAR-T cells were co-cultured with LK2-hGPC1 (a hGPC1 overexpressing lung carcinoma cell line), TE14 (an esophageal carcinoma cell line with endogenous hGPC1), or LK2-mock (a hGPC1-negative lung carcinoma cell line), IFNγ secretion and Cr51 release were detected in hGPC1-dependent manner (Figure 2B,C and D). Here, GPC1 is linked to carcinoma of esophagus.