WNT5A and colorectal carcinoma: To clarify the functions of Wnt5a-induced macrophages in CRC in vitro, M0 macrophages were first treated with Wnt5a with or without IL-10 antibody or IgG for 72 h, and then co-cultured with HCT116 or DLD-1 cells for another 36 h, which were harvested for subsequent experiments (Fig. 5a).