Univariate survival analysis was then performed using the R-package survival, and the results indicated that CNFN was associated good survival (Fig. 6a) but KIF18B, KIF23, PRC1, CCNA2, DEPDC1, and TTK were associated with poor survival in HNSCC samples with LOI (P < 0.05; Fig. 6c–h). Here, CCNA2 is linked to head and neck squamous cell carcinoma.