We used Western blotting to detect the expression level of ANXA1 in breast cancer cells (BT-549, MDA-MB-231, MCF7, and T-47D) and found that ANXA1 level was significantly higher in BT-549 and MDA-MB-231 cells (highly invasive) than in MCF7 and T-47D cells (low invasive) (Figure 4B), which was consistent with the expression of DCST1-AS1 (11). This evidence concerns the gene DCST1 and breast cancer.