Moreover, compared to HF-fed WT mice, HF-fed Egr1-/- mice had higher phosphorylation levels of IRS1 Tyr896 and Akt Ser473 in the liver (Figure S2B), and higher energy expenditure, as measured by oxygen consumption (VO2) and carbon dioxide production (VCO2) during both dark and light phases (Figure S2C). Here, IRS1 is linked to hydrops fetalis.