Expression levels of genes encoding members of the hepatocyte nuclear factor family (HNF1A, HNF1B, HNF3B, and HNF4A), which are required for proper pancreatic development 11, 36, were significantly downregulated in the islets of HF-fed Egr1-/- mice (Figure 7B). This evidence concerns the gene EGR1 and hydrops fetalis.