These results showed that ERRα overexpression exhibited a positive correlation with the up-regulation of two steroidogenic rate-limiting enzyme genes, CYP11A1 and AKR1C3, involved in androgen biosynthesis in prostate cancer cells, suggesting that ERRα could function to promote the castration-resistant growth via the up-regulation of steroidogenic enzymes involved in androgen biosynthesis. This evidence concerns the gene AKR1C3 and prostate carcinoma.