Immunoblot analysis of AR and its target PSA in prostate cancer cells showed that supplement with an androgen precursor 5α-Adione could enhance the levels of nuclear AR level (without change in total AR level) and PSA in both LNCaP-pBABE and LNCaP-ERRα transduced cells, with significant higher levels in LNCaP-ERRα cells (Figure 7A). The gene discussed is AR; the disease is prostate carcinoma.