Four groups of ESCC cells were set: ESCC cells transfected with NC, ESCC cells transfected with miR-34a-5p mimics, ESCC cells transfected with LEF1 expression plasmid, and ESCC cells co-transfected with miR-34a-5p and LEF1-expressing plasmid. The gene discussed is LEF1; the disease is esophageal squamous cell carcinoma.