To investigate the biological effects of LEF1 in ESCC cells, we applied a lentivirus-based approach to stably express LEF1 (ov-LEF1) or shLEF1 (sh-LEF1) in EAC109 and TE1 cells, and the expression level of LEF1 was examined by qRT-PCR and western blotting. Here, LEF1 is linked to esophageal squamous cell carcinoma.