To examine the effect of icotinib plus BDMC in EGFR-TRI-resistant NSCLC cells with different EGFR characteristics, three primary TKI-resistant cell lines, including A549, H460 and H1781 were used, which showed an overall pattern of increased resistance when incubated with icotinib for 48 h (IC50 > 20 μM for the three cell lines), compared with TKI-sensitive cell line HCC4006 (IC50 = 55.4 nM) (Figure 1A). Here, EGFR is linked to non-small cell lung carcinoma.