SQSTM1 and neoplasm: Immunoblot assay showed that combination treatment clearly upregulated expressions of LC3-, Beclin-1, cleavad-caspase 7 and -PARP, and downregulated SQSTM1 expression compared to single treatment in xenograft tumor specimens (Figure 7D), which were in consistent with the finding in vitro (Figure 4C and 4F).