CD46 and infection: Figure 1C,D demonstrate an increase in median CD46 and DSG2 expression 24 h post irradiation of A549 cells. We therefore used qPCR to quantify EnAd uptake in cells infected 24 h post 10 Gy of radiation. We did not observe any differences between irradiated and non-irradiated cells infected with EnAd-SA-GFP at MOI 0.1 or mock-infected (Figure 1E). These results suggest the increase in virus production seen in Figure 1A is not due to enhanced virus uptake during a multi-step infection and are consistent with receptor level not being a limiting step in virus propagation at this MOI.