Representative histology of all treatments can be seen in Figure 1B. To determine if RMS feeds were inducing inflammation, serum samples were collected at the end of the experiment and measured for Infγ, IL-10, IL-1β, IL-6, KC-GRO (murine homologue of IL-8), and TNF, which are cytokines often associated with intestinal injury and human NEC [61]. This evidence concerns the gene CALCA and necrotizing enterocolitis.