Their maturation requires mechanosensitive influx of Ca2+ and activation of downstream Ca2+∕calmodulin-dependent kinase kinase 2 (CaMKK2)-AMP-activated protein kinase (AMPK)-AMPK-vasodilator-stimulated phosphoprotein (VASP) signaling cascade, at least in human osteosarcoma cells (Tojkander et al., 2015, Tojkander et al., 2018). Here, CAMKK2 is linked to osteosarcoma.