Tcm (CD3+CD62L+CD45RO+) cells cultured ex vivo by the Newish company after isolation from peripheral blood mononuclear cells (PBMC) of hepatocellular carcinoma (HCC) patients could effectively kill the human HCC cell line, QGY-7703, while relatively high levels of IFN-γ and TNF-α were secreted in the process of Tcm activation. Here, TNF is linked to hepatocellular carcinoma.