Furthermore, 3′-SL regulates the expression of cytokines produced by Th1 cells (IFN-γ, TNF-α), Th2 cells (IL-4, IL-5, IL-13) and Th17 cells (IL-17) by blocking the intranuclear translocation of p65 in the ear tissue of HDM and 1% DNCB induced AD model mice. This evidence concerns the gene TNF and Alzheimer disease.