For example, it was found that bufalin treatment resulted in the down-regulation of Wnt/Achaete-scute-like 2 expression in gastric cancer [31], HIF-1α expression via inhibition of the phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B/mammalian target of rapamycin pathway in hepatocellular carcinoma [50], and heat shock protein-27 expression in osteosarcoma cells [51]. The gene discussed is HIF1A; the disease is osteosarcoma.