SEMA3D and Hirschsprung disease: Furthermore, qPCR showed that the SEMA3D expression was strongly up-regulated (5.5-fold) (Figure 2) in the ganglionic colon of HSCR patients compared to control colon (ΔCT 10.8 ± 2.1 vs. 13.3 ± 3.9; p = 0.025) (Table 4, Figure 3), while the SEMA3D expression was not significantly different between the aganglionic colon of HSCR patients and the control colon (ΔCT 13.1 ± 3.0 vs. 13.3 ± 3.9; p = 0.89) (Table 4, Figure 3).