To meet these challenges, in a previous study we have designed, developed, and validated, for both tissue samples and liquid biopsy specimens, a narrow NGS gene panel (SiRe®) that covers 568 clinically relevant mutations in six genes (EGFR, KRAS, NRAS, BRAF, cKIT, and PDGFRα) involved in NSCLC, gastrointestinal stromal tumor, mCRC, and melanoma (19–23). The gene discussed is EGFR; the disease is gastrointestinal stromal tumor.