Because gene sets with the highest enrichment scores were more closely associated with HF growth in high-expression groups of Tβ4 and ARHGAP6, we further validated the RNA-seq results of Tβ4 and ARHGAP6. Combined with some reports [30,36,43], we chose Tβ4 as the target gene for the study. This evidence concerns the gene ARHGAP6 and hydrops fetalis.