Because gene sets with the highest enrichment scores were more closely associated with HF growth in high-expression groups of Tβ4 and ARHGAP6, we further validated the RNA-seq results of Tβ4 and ARHGAP6. Their expression patterns in anagen and telogen stages of the HF were examined using quantitative real-time PCR (qPCR). The gene discussed is ARHGAP6; the disease is hydrops fetalis.