Ex vivo restimulation of splenocytes with growth-arrested tumor cells again demonstrated a significant enhancement of tumor-specific IFNγ production up to 2 weeks after mice were treated with a single dose of VVΔTKΔN1L (figure 3B), increased activation and cytotoxicity of CD8+ T cells (figure 3C; online supplementary figure S2C) and increased infiltration of CD4+ and CD8+ T cells into the tumor (figure 3D). This evidence concerns the gene CD4 and neoplasm.