In this study, we identify hepatocyte nuclear factor 4 alpha (HNF4A) as a transcription factor facilitating aerobic glycolysis and NB progression, and reveal that HNF4A antisense RNA 1 (HNF4A-AS1), a lncRNA derived from upstream region of HNF4A, is associated with poor outcome of NB. Here, HNF4A-AS1 is linked to neuroblastoma.