Importantly, blocking HNF4A-AS1-hnRNPU interaction or lentivirus-mediated HNF4A-AS1 knockdown was able to suppress aerobic glycolysis, tumorigenesis, and aggressiveness of NB cells, suggesting the oncogenic roles of HNF4A-AS1/hnRNPU/CTCF axis in aerobic glycolysis and tumor progression. This evidence concerns the gene CTCF and neuroblastoma.