This study has shown that ANP expression would be upregulated under myocardial ischemia and reperfusion injury in normal rats, but it was not enough to resist myocardial insult resulted from ischemia reperfusion in diabetic rats, while postconditioning could not increase the expression of APN and p-Akt after MIR injury. This evidence concerns the gene AKT1 and myocardial ischemia.