BRAF and melanoma: To examine whether DLC1 has a role in regulating melanoma growth and metastasis, we performed gene KD by designing two different shRNA lentiviral constructs to stably silence DLC1 transcripts (DLC1 KD1 and DLC1 KD2) in BRAF-mutated A375 and WM266-4 cell lines, as they showed high levels of DLC1 expression (Fig. 1g).