DLC1 and melanoma: However, cytoplasmic DN-DLC1 was ineffective in inhibiting melanoma invasion, antagonizing the oncogenic functions of ectopic WT-DLC1 and K714E-DLC1 in UACC-827 cells (Supplementary Fig. 4f), and restoring migration defects of DLC1 KD A375 and WM266-4 cells (Supplementary Fig. 4g, h), ruling out the oncogenic role of cytoplasmic DLC1.