DLC1 and melanoma: To further investigate whether the observed increase in DLC1 expression levels in cutaneous melanomas functionally promotes their progression, we overexpressed (OE) wild-type DLC1 (WT-DLC1) and K714E-DLC1 (RhoGAP deficient mutant) [22] in A375 and WM266-4 melanoma cells (Supplementary Fig. 2a).