Moreover, enforced expression of WT-DLC1 or K714E-DLC1 did not further enhance colony formation, proliferation, and invasiveness of melanoma cells (Supplementary Fig. 2c–f), whereas WT-DLC1 but not K714E-DLC1 inhibited colony formation, proliferation, and invasion of DU145 cells consistent with the tumor-suppressor properties of DLC1 acting through its RhoGAP activity in prostate cancer cells (Supplementary Fig. 2g–i) [27]. This evidence concerns the gene DLC1 and Familial prostate cancer.