To overcome the challenges mentioned earlier on use of the mdx mouse, particularly with regard to its mild phenotype, numerous groups have developed DMD mouse models of their own by either crossing the mdx mutation to other strains of mice, using chemical mutagenesis to generate new mutations in other regions of the Dmd gene, or by generating targeted disruptions or a total knockout of the gene [24]. Here, DMD is linked to Duchenne muscular dystrophy.