Therefore, we verified the correlations between the tumour immune microenvironment and eight representative immune checkpoint genes, including PDCD1, CD274, PDCD1LG2, CTLA4, CD86, CD80, CD276, and VTCN1. The results showed that there was a significant positive correlation between immune microenvironment genes and all the immune checkpoint genes other than VTCN1. This suggested that these immune microenvironment genes might be potential immunotherapeutic targets. The gene discussed is CTLA4; the disease is neoplasm.