In the validation of protein expression encoded by the genes using clinical specimens from Human Protein Profiles, we found that TMC8 was strongly positive, BIN2, GIMAP7, and SPOCK2 were moderately positive, and FYB1, RCSD1, and SASH3 were weakly positive in HCC tissues relative to their expression levels in normal liver tissues (Figure 11B). This evidence concerns the gene TMC8 and hepatocellular carcinoma.