To confirm transmembrane localization in neuroblastoma, we first performed a membrane extraction in four MYCN amplified neuroblastoma cell lines [KELLY, CHP-134, LA-N-5, and SK-N-BE (2)c], three non-amplified neuroblastoma cell lines (NB-Ebc1, SK-N-AS, SK-N-FI) and RPE-1 cells. This evidence concerns the gene MYCN and neuroblastoma.