MYCN and neuroblastoma: To investigate whether MYCN protein regulates CAMKV expression, we transiently depleted MYCN from MYCN amplified IMR-05 cells using shRNA and saw a significant reduction in CAMKV mRNA levels (Figure 4A, p < 0.005), suggesting that MYCN is required for CAMKV transcription in MYCN amplified neuroblastoma cells.